Table 1. Deduced functions of the ORFS identified in this work.
| Protein | Size, aa | Proposed function | Closest homolog, protein (origin) | S/I, %/% |
|---|---|---|---|---|
| TP | 140* | Transposase | MM1044 (M. mazei) | 72/52 |
| OnnA | 420 | HMGS-like activity | PksG (B. subtilis) | 85/71 |
| PedP (Pa. fuscipes symbiont) | 76/63 | |||
| OnnB | 4,359† | PKS (domains: GNAT, ACP, KS, KR, MT, ACP, KS, CR, CR, ACP, ACP, ACP‡, KS, ACP) | Pedl (domains: GNAT, ACP, KS, KR, MT, ACP, KS, CR, CR, ACP, ACP, KS, ?, KR, ACP) | 63/46 |
| OnnC | 386 | Oxygenase | PedJ | 81/65 |
| OnnD | 317 | MT | PedA | 68/50 |
| OnnE | 356 | Unknown | PedK | 66/50 |
| OnnF | 340 | Unknown | PedK | 71/55 |
| OnnG | 321 | MT | PedA | 70/53 |
| OnnH | 268 | MT | PedE | 71/52 |
| Onnl | 5,027* | PKS (domains: KS‡, ACP, C, A, PCP, KS, KR, ACP, KS, KR, MT, ACP, ACP, KS) | PedF (domains: KS‡, ACP, C, A, PCP, KS, KR, ACP, KS, KR, MT, ACP, KS, DH, DH, KR, ACP, KS, KR, ACP, KS, DH‡ | 64/49 |
| PedL | 251 | CR superfamily | PksH (B. subtilis) | 69/53 |
| PedM | 443 | KS | PksF (B. subtilis) | 56/35 |
| PedN | 80 | ACP | AcpK (B. subtilis) | 57/41 |
| PedO | 403 | MT | PedA | 68/54 |
| PedP | 430 | HMGS-like activity | PksG (B. subtilis) | 79/65 |
| OnnA (T. swinhoei symbiont) | 76/63 | |||
| PedQ | 314 | Esterase | PA2949 (Ps. aeruginosa) | 47/30 |
| PedR | 297 | LysR-type regulator | PA0784 (Ps. aeruginosa) | 64/49 |
onn genes were from T. swinhoei, and ped genes were from Pa. fuscipes. S/I, protein similarity/identity; M. mazei, Methanosarcina mazei; B. subtilis, Bacillus subtilis; GNAT, GCN5-related N-acetyltransferase; C, condensation; A, adenylation; PCP, peptidyl carrier protein; DH, dehydratase.
*
Fragment.
†
Size of 4,376 aa when measured from alternate start codon.
‡
Domain lacking active site motifs.