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. 2017 Feb 2;12(2):e0171161. doi: 10.1371/journal.pone.0171161

Fig 7. Nrf2 deletion increases bone remodeling markers or bone formation markers in female or male Nrf2 KO mice, respectively, without significantly altering tissue bone formation indexes.

Fig 7

Circulating levels of alkaline phosphatase (ALP), procollagen type 1 amino-terminal propeptide (P1NP) and C-telopeptide fragments (CTX) were measured in female (A) and male (B) WT and Nrf2 KO mice at the indicated ages. Bars are means ± S.D, n = 8–10 mice/ group. # p<0.05 by t- Test. Dynamic (C) and static (D) histomorphometric parameters were scored in L1-L3 lumbar vertebral bone sections of 15-month-old WT and Nrf2 KO male mice. Mineralizing surface (MS)/BS, mineral apposition rate (MAR), and bone formation rate (BFR)/BS were quantified in unstained sections. Osteoid volume (OV)/BS, number of osteoblasts (N.Ob)/BS, and number of osteocytes (N.Ot)/ B.Ar were measured in sections stained with von Kossa and counterstained with toloudine blue. Bars are means ± S.D, n = 6–8 mice/ group. * p<0.05 versus respective WT by two-way ANOVA, followed by pairwise multiple comparisons using Tukey method.