Table 1. Longitudinal analysis of BMD in KO mice and WT littermates.
In this model, b0+b1 corresponds to BMD at age = 1 month, b1 represents the curvature of the relationship from 1 month up to b2, b2 is the age at peak bone mass, and b3 is the slope of the relationship for age greater than b2. Two-way significance tests (main effects of gender and genotype and the gender by genotype interaction) for each parameter were performed. Estimates and standard errors of BMD at age 1 month (b0+b1), curvature (b1), age at peak bone mass (b2), slope after peak (b3), BMD at peak ((b0+(b1/b2)), and BMD at age 15 months ((b0+(b1/b2)+b3*(15-b2)) were calculated.
| A Total | Females | Males | ||
| WT | Nrf2 KO | WT | Nrf2 KO | |
| BMD at 1 month (b0+b1) | 0.0338 ± 0.0004 | 0.0320 ± 0.0005 a | 0.0338 ± 0.0004 | 0.0348 ± 0.0005 |
| Curvature (b1) | -0.0246 ± 0.0006 | -0.0246 ± 0.0006 | -0.0246 ± 0.0006 | -0.0246 ± 0.0006 |
| Age at peak bone mass (b2) | 5.1071 ± 0.4828 | 7.0971 ± 0.6366 b | 3.3311 ± 0.2169 | 3.5002 ± 0.2594 |
| Slope after peak (b3) | 0 | 0 | 0 | 0 |
| BMD at peak (b0+(b1/b2) | 0.0536 ± 0.0003 | 0.0532 ± 0.0002 | 0.0510 ± 0.0002 | 0.0524 ± 0.0001 a |
| BMD at 15 months (b0+(b1/b2))+b3*(15-b2)) | 0.0536 ± 0.0003 | 0.0532 ± 0.0002 | 0.0519 ± 0.0002 | 0.0463 ± 0.0001 a |
| B Femur | Females | Males | ||
| WT | Nrf2 KO | WT | Nrf2 KO | |
| BMD at 1 month (b0+b1) | 0.0397 ± 0.0115 | 0.0368 ± 0.0011 a | 0.0397 ± 0.0013 | 0.0426 ± 0.0013 a |
| Curvature (b1) | -0.0366 ± 0.0006 | -0.0366 ± 0.0006 | -0.0443 ± 0.0022 | -0.0443 ± 0.0022 |
| Age at peak bone mass (b2) | 7.2510 ± 0.1502 | 9.3564 ± 0.1533 b | 3.4760 ± 0.2989 | 3.4184 ± 0.3052 |
| Slope after peak (b3) | 0 | 0 | 0 | 0 |
| BMD at peak (b0+(b1/b2) | 0.0713 ± 0.0005 | 0.0695 ± 0.0005 a | 0.0714 ± 0.0003 | 0.0740 ± 0.0005 a |
| BMD at 15 months (b0+(b1/b2))+b3*(15-b2)) | 0.0713 ± 0.0005 | 0.0695 ± 0.0005 a | 0.0714± 0.0003 | 0.0740 ± 0.0005 a |
| C Spine | Females | Males | ||
| WT | Nrf2 KO | WT | Nrf2 KO | |
| BMD at 1 month (b0+b1) | 0.3823 ± 0.0009 | 0.0358 ± 0.0010 a | 0.3823 ± 0.0009 | 0.0358 ± 0.0010 a |
| Curvature (b1) | -0.0312 ± 0.0015 | -0.0312 ± 0.0015 b | -0.0312 ± 0.0015 | -0.0312 ± 0.0015 b |
| Age at peak bone mass (b2) | 4.6757 ± 0.4935 | 6.2914 ± 0.5845 | 2.3018 ± 0.1504 | 3.9176 ± 0.3261 |
| Slope after peak (b3) | 0.0007 ± 0.00008 | 0.0007 ± 0.00008 | 0.0007 ± 0.00008 | 0.0007 ± 0.00008 |
| BMD at peak (b0+(b1/b2)) | 0.0628 ± 0.0006 | 0.0621 ± 0.0005 | 0.0559 ± 0.0006 | 0.0576 ± 0.0006 b |
| BMD at 15 months (b0+(b1/b2))+b3*(15-b2)) | 0.0550 ± 0.0008 | 0.0544 ± 0.0007 | 0.0481 ± 0.0006 | 0.0499 ± 0.0008 b |
a p<0.05 vs respective WT
b p<0.001 vs respective WT