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. 2004 Nov 1;101(46):16210–16215. doi: 10.1073/pnas.0405984101

Fig. 6.

Fig. 6.

Netrin-1 is synergistic with VEGF in both the in vivo corneal micropocket assay and the in vitro migration assay. (A and B) Netrin-1 induced angiogenesis in a murine corneal micropocket assay. Hydron pellets containing equimolar amounts of either Netrin-1 (200 ng) or VEGF (50 ng) stimulated comparable levels of blood vessel growth. When combined, the two factors generated an accentuated response that was greater than the sum of the responses to each factor individually. In a single experiment, each test factor was placed on six corneas. Each experiment was repeated a minimum of three times. Quantitative assessment and visual representations are shown. (C) Treatment of endothelial cells with Netrin-1 and VEGF together synergistically enhances migration. Either 10 ng/ml Netrin-1 or 2.5 ng/ml VEGF alone showed a <2-fold increase over BSA control. However, when 10 ng/ml Netrin-1 and 2.5 ng/ml VEGF were combined, the response was greater than the sum of each factor treated separately. The asterisks indicate treatments that yield a statistically significant increase (P < 0.05, Student's t test) in angiogenesis. The double asterisks indicate treatment that yields a statistically significant increase (P < 0.001) over individual Netrin-1- or VEGF-treated samples.