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. 2017 Feb 2;13(2):e1006164. doi: 10.1371/journal.ppat.1006164

Fig 2. Differential infection of human DCs by evolutionarily distinct ZIKV strains.

Fig 2

moDCs were infected with PR-2015, P6-1966, MR-1947, or Dak-1984 at MOI of 1 and assessed for viral replication at the indicated hours post-infection. (A) Infectious virus release into the supernatant was determined by FFA. Shown as the mean +/- SEM from 6–9 donors. (B) Infectious virus release for 6 of the individual donors summarized in panel A. (C) Percent infected cells assessed by ZIKV E protein staining and flow cytometry. Shown as the mean +/- SEM from 6–9 donors. (D) Percent infected cells in 6 of the individual donors summarized in panel C. (E) Cell viability of infected moDCs assessed by Ghost Red 780 (Tonbo) viability staining and flow cytometry. Shown as the mean +/- SEM from 6–9 donors. Statistical significance (p< 0.05) was determined using a two-way ANOVA with comparisons made to mock-infected cells. See also S1 Table.