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. 2017 Feb 2;13(2):e1006164. doi: 10.1371/journal.ppat.1006164

Fig 8. ZIKV antagonizes type I IFN signaling.

Fig 8

(A, B) A549 cells were infected with PR-2015, P6-1966, MR-1947, or Dak-1984 at MOIs of 0.1 and 1. At 48hpi, cells were pulse treated with 1000 IU/mL of recombinant human IFNβ for 30 minutes and whole-cell lysates were collected for western blot analysis of phospho-STAT1 (Tyr701), phospho-STAT2 (Tyr689), STAT1, STAT2, and GAPDH. Representative blots are shown from one of two independent experiments. Quantitation is shown below the representative blots, where intensity values are represented as the ratio of pSTAT:total STAT protein. (C) moDCs were infected with PR-2015 (MOI 10) and STAT1 and STAT2 signaling was assessed as in A and B. Data is representative of three donors from two independent experiments. Quantitation is shown to the right of the representative blots, where intensity values are represented as the ratio of pSTAT:total STAT protein.