Fig 3. PP1 functions with I-2^SZY-2 and SDS-22 to regulate centriole duplication.

A) Comparison of phospho-HH3 levels in control and PP1β^GSP-1 & PP1α^GSP-2 co-depleted embryos as determined by quantitative immunofluorescence microscopy. The mean is indicated by the bar. B) Western blot demonstrating specificity of gsp-1(RNAi). C&D) Stills from time-lapse recordings of embryos of the indicated genotypes, expressing GFP::histone and GFP::SPD-2 grown at the normally restrictive temperature of 24°C. E&F) Stills from time-lapse recordings of embryos of the indicated genotypes, expressing mCherry::histone and GFP::tubulin grown at the normally restrictive temperature of 24°C; centrosomes are indicated by arrow heads. G) Quantitation of centriole duplication in indicated strains. Number of centriole duplication events scored is indicated. H) Western blots of immunoprecipitated material from whole worm extracts using control IgG, I-2^SZY-2, or PP1β^GSP-1 antibodies, and probed with I-2^SZY-2, PP1β^GSP-1, or SDS-22 antibodies as indicated. I) Western blots of immunoprecipitated material from whole worm extracts using control IgG, I-2^SZY-2, or SDS-22 antibodies, and probed with I-2^SZY-2 or PP1α^GSP-2, or SDS-22 antibodies.