Table 1. Gene-specific primers designed for the designated mouse genes (Genbank ID) and used to determine the expression levels by RT-qPCR.
| Name | Genbank ID | Sequence (5'->3') | product length (bp) |
|---|---|---|---|
| GAPDH | gi|53237094| | TTGCAGTGGCAAAGTGGAGA | 175 |
| GTCTCGCTCCTGGAAGATGG | |||
| Bcl-2 | gi|929981607| | GAACTGGGGGAGGATTGTGG | 176 |
| CCAGACATGCACCTACCCAG | |||
| Bax | gi|133778943| | CACTAAAGTGCCCGAGCTGA | 167 |
| TGAGGACTCCAGCCACAAAG | |||
| Cyclin E | gi|443939| | TATGGTGTCCTCGCTGCTTC | 202 |
| GGGTCTGGATGTTGTGGGAG | |||
| CDK2 | gi|162287299| | GTGGTACCGAGCACCTGAAA | 188 |
| CTGGCCAAACCACCTCATCT | |||
| p21 | gi|50300976| | TTGCACTCTGGTGTCTGAGC | 190 |
| GGGAAGGGCTGGAATGTTCT |