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Coexpression of SV5-Δ2 or SV5-Δ4 mutants with activators Δ1/Δ3 and Δ3. (A) Schematic representation of NSP5 and the deletion mutants constructs. (B) Coexpression assay in MA104 cells of SV5-Δ2 (lanes 1-3) or SV5-Δ4 (lanes 4-6) substrates with or without the indicated activators. (C) λ-Ppase treatment of SV5-Δ2 cotransfected with activator Δ3. Samples were loaded in a 15% SDS/PAGE, and substrates were visualized by Western blot.
