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Activator-dependent wt NSP5 phosphorylation. (A) Coexpression in MA104 cells of wtNSP5 or NSP5a with the activator Δ1/Δ3. Immunoblot developed with anti-NSP5 serum. The arrow indicates the position of Δ1/Δ3. Bracket indicates NSP5 phosphorylated forms. (B) Expression of full-length wtNSP5 and NSP5 point mutants. Lane 5 corresponds to NSP5 from rotavirus-infected MA104 cells. Samples were migrated in a 15% SDS/PAGE and visualized by Western blot.
