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. 2004 Nov 1;101(46):16304–16309. doi: 10.1073/pnas.0406691101

Fig. 5.

Fig. 5.

NSP5 phosphorylation by CK1. (A) Time course of CK1α phosphorylation of motif a peptides derived from NSP5. Phosphorylations were carried out as described in Materials and Methods by using peptides at 200 μM concentration and recombinant zebrafish CK1α. The amino acidic sequence of both peptides is indicated. NSP5wt (•) and NSP5(S67A) (○). (B) SDS/PAGE autoradiography of in vitro phosphorylation assay of mutants Δ3, Δ3/S67A, and Δ3a (Left) or wtNSP5,NSP5/(S63,65A/S67D) and NSP5a (Right), with recombinant CK1α and [32P]-γATP. The 38-kDa band corresponds to autophosphorylated CK1α. Reactions were carried out at the indicated concentrations of substrate proteins. Negative control reactions containing either CK1α alone (lanes 1 and 9) or substrate Δ3 alone (lane 8) are shown.