Table 1.
Age of mice at experimental time points.
| Baseline | Insult | Post-insult measurements | |
|---|---|---|---|
| Colorectal distension | |||
| No insult (n = 10) | 13 | ||
| Saline (n = 6) | 8 | 9 | 10 |
| TNBS 2 mg (n = 6) | 10 | 11 | 12 |
| TNBS 5 mg (n = 6) | 8 | 9 | 10 |
| 1d WAS (n = 6) | 10 | 10 | 11 |
| 7d WAS (n = 6) | 10 | 10–11 | 12 |
| Open Field | |||
| CRD (n = 8) | 6 | 13 | 17 |
| Hindpaw Sensitivity | |||
| CRD (n = 8) | 7 | 13 | 19 |
Naïve and NMS mice underwent behavioral or physiological testing at the above noted ages (in weeks) prior to and/or following introduction of an insult, including colorectal distension (CRD), intracolonic trinitrobenzene sulfonic acid (TNBS), or acute (1d) or chronic (7d) exposure to water avoidance stress (WAS). Repeated tests were performed on the same mice for all CRD experiments involving post-insult measurements. Open field and hindpaw sensitivity tests were performed on separate groups of mice either at baseline or following CRD.