Fig. 4.

Osteocytes and myeloid cells from old chronologically aged mice develop the senescence-associated secretory phenotype (SASP). (A) Osteocytes and (B) myeloid cells were rapidly isolated from young (age 6 months; n = 12) and old (age 24 months; n = 10) male mice (without in vitro culture). In vivo age-associated changes in normalized mRNA expression of 36 established SASP components are shown. Results are expressed as mean ± SE. NE = not expressed (Cycle threshold [Ct] values >35 in both young and old samples). ^*p < 0.05; ^**p < 0.01; ^***p < 0.001.