Fig. 3.

EB virion proteins before and after deenvelopment and virion phosphoproteins. (A) Purified EBV proteins were separated by SDS-polyacrylamide gels directly (lane E) or after treatment with NP-40 (lane N) or ND (lane ND) and stained with Sypro-Ruby. Molecular sizes are given in parenthesis. (B) Purified EB virion proteins were separated on SDS-polyacrylamide gels and stained with Sypro-Diamond to detect phosphoproteins (lane PP). The same gel was restained with Sypro-Ruby to visualize all virion proteins (lane P). BKRF4 is in small type because of its low abundance in virions and phosphopeptides from two host-encoded proteins (protein kinase A and neural-encoded death-determining protein 5) were also detected at 42 kDa (Table 6).