Table 1. Proteins detected in 10 μg of deglycosylated EBV.

					Peptides
Protein type	EBV ORF	HSV UL	Function	Coverage, %	Total, n	Unique, n	Area, units	MWobs, kDa
Capsid	BcLF1†	19†	MCP	67	112	62	23,628	155
	BDLF1	18†	mCP	49	25	11	3,894	30
	BFRF3	35†	sCP	64	35	12	3,205	18
	BORF1	38†	mCPBP	40	17	9	1,576	40
	BBRF1	6†	Portal	34	14	14	300	68
	(BdRF1)	26.5	Scaffold	6	2	2	13	?
Glycoprotein	BLLF1†	γ1	gp350	29	31	15	4,067	350
	BXLF2†	22†	gH	41	30	18	1,344	88
	BALF4	*	gB-N	54	22	14	1,014	78
	BALF4	*	gB-C	50	25	21	966	58
	BALF4†	27†	gB-FL	40	21	20	372	120
	BZLF2†	γ	gp42	33	9	6	286	42
	BBRF3	10†	gM	17	4	4	74	?
	BILF2	γ1	gp78	10	2	2	54	DG
	BLRF1	49.5†	gN	8	1	1	40	DG
	BDLF3†	γ	gp150	15	3	3	34	115
	BKRF2†	1†	gL	18	2	1	21	25
	BMRF2†	γ	epi ligand	3	1	1	21	DG
Tegument	BPLF1	36†	LTP	39	108	87	3,307	350
	BGLF2	16†	MyrPBP	67	19	12	1,021	32
	BOLF1	37†	LTPBP	31	25	24	944	140
	BVRF1	25†	Capsid-assoc.	45	20	17	656	58
	BBLF1	11†	MyrP	87	16	4	430	15‡
	BGLF1	17†	Packaging	23	6	6	122	58
	BSRF1	51†	PalmP	19	5	4	120	27‡
	BGLF4	13†	TS kinase	25	7	7	109	47
	BNRF1†	γ	MTP	54	87	51	15,845	140
	BLRF2†	γ	Unknown	64	35	12	3,205	19
	BRRF2	γ	Unknown	73	34	23	3,152	72‡
	BDLF2	γ	Unknown	32	13	10	304	54
	BKRF4	γ	Unknown	18	7	3	219	42‡
	BORF2§	39	RNR-L	35	19	19	249	90
	BALF2§	29	ssDNABP	25	16	16	227	130
	BXLF1§	23	TK	14	10	7	145	72
	BMRF1§	42	dsDNABP	25	7	7	118	49
	Host	N/A	Actin	70	63	22	10,022	45
	Host	N/A	Hsp70	61	54	33	2,713	72
	Host	N/A	Cofilin	69	37	15	2,670	17
	Host	N/A	β-Tubulin	74	37	22	2,290	54
	Host	N/A	Enolase	54	30	22	1,402	49
	Host	N/A	Hsp90	53	48	38	1,394	90

Coverage as a percentage of ORF, total number of detected peptides, number of unique peptides, total peptide area for all peptides assigned to each protein, and observed molecular sizes are shown from the sequencing of 10 μg of partially deglycosylated EB virion proteins. The observed molecular mass (MW_obs) for glycoproteins is from nondeglycosylated virion protein sequencing experiments. Corresponding HSV ORF (HSV UL) and putative function are also shown. γ and γ1 refer to proteins that have only γ- or γ1- herpesvirus homologues. ?, protein was detected over a range of sizes; DG, glycoprotein was only detected in deglycosylated state; N/A, not applicable.

^*HSV UL27 (gB) does not undergo cleavage into N- and C-terminal polypeptides.

^†Known virion protein.

^‡Nonglycoprotein with observed molecular size deviating > 10% from that predicted.

^§Highly abundant infected cell proteins; significance discussed in text.