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. Author manuscript; available in PMC: 2017 Feb 2.
Published in final edited form as: Biochemistry. 2015 Oct 21;54(43):6576ā€“6585. doi: 10.1021/acs.biochem.5b00664

Figure 7. NMR titrations of PcrG and PcrV.

Figure 7

(A) Six overlaid 2D 1H-15N HSQC spectra of 15N-labeled PcrG9ā€“76 titrated with increasing amounts of PcrV. (B, C) Two overlaid 2D 1H-13C HSQC spectra of isoleucine 13CĪ“1 methyl labeled PcrV in complex with (B) PcrG and (C) PcrG9ā€“76 in a 1:1 ratio. The slow-exchange peaks (boxed), and peaks in fast-exchange (arrows) are indicated.