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Expression and purification of RGS9 constructs. (a) Coomassie-stained SDS/PAGE gel of N-terminally His6-tagged purified RGS9 constructs. Preparations were >90% pure. (b) Schematic diagram of RGS9 constructs. Domains are DEP (Dishevled/Egl10/Plextrin), GGL (G gamma-like), RGS (Regulator of G protein signaling), and PSR (proline/serine-rich).
