Figure 4.

The effects of Gcn2 abolishment on Gln3-Myc¹³ localization in response to short- and long-term nitrogen starvation. (A) Wild-type (BY4742) and (B) gcn2Δ cells were grown to A_{600 nm} = 0.5 in YNB-glutamine medium. They were then transferred (by filtration) to nitrogen-free YNB medium and sampled as indicated for 6 hr. At that time glutamine (0.1%) was added to the culture and sampling continued for 30 min. Gln3-Myc¹³ was visualized and scored as described in Materials and Methods. Nucl.-Cyto., nuclear-cytoplasmic.