Figure 5.

The effects of Gcn2 abolishment on budding and Gln3-Myc¹³ localization in response to long-term nitrogen starvation. (A) Wild-type (BY4742) and (B) gcn2Δ cells were grown to A_{600 nm} = 0.5 in YNB-glutamine medium. They were then transferred (by filtration) to nitrogen-free YNB medium and sampled as indicated for 10 hr. Gln3-Myc¹³ was visualized and scored as described in Materials and Methods. The extent of cell budding (C) was scored in 200 or more cells per determination. Nucl.-Cyto., nuclear-cytoplasmic.