Figure 3.

Global discovery of mutations within the TA of a Gal4–sfGFP–Fis1 fusion protein that allow Gal4p-driven transcription. The log₂ of enrichment values for each amino acid were calculated for each position following selection in SMM −Trp −His medium containing 20 mM 3-AT. Enrichment values are generated for individual amino acid positions within the TA and not across positions. Black outlines denote the native amino acid at each position. Amino acid replacements not detectable under selective conditions are denoted by black, filled squares. The predicted MAD is indicated by a red line. “X” represents substitution by a stop codon.