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. 2016 Dec 18;205(2):691–705. doi: 10.1534/genetics.116.196428

Figure 5.

Figure 5

Proline substitution is acceptable at a discrete position within the Fis1p TA. (A) Replacement of specific amino acids within the TA of Gal4–sfGFP–Fis1p with proline can lead to Gal4p-mediated selectable marker activation. Strain MaV203, expressing Gal4–sfGFP–Fis1p variants from plasmids b100 (WT), b188 (V134P), b189 (G137P), b129 (L139P), b190 (A140P), b296 (A144P), or b101 (∆TA), was cultured in SC −Trp medium and then spotted to SC −Trp or SMM −His + 20 mM 3-AT medium for 2 days. (B) TAs with specific proline replacements can reduce mitochondrial targeting of a linked fluorescent protein. Variants of the Fis1p TA fused to mCherry were expressed in WT strain CDD961 from plasmids b109 (WT), b208 (V134P), b209 (G137P), b135 (L139P), b210 (A140P), b211 (A144P) and examined, along with mitochondria-targeted GFP, as in Figure 2B. Bar, 5 µm.