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. 2016 Dec 18;205(2):691–705. doi: 10.1534/genetics.116.196428

Figure 6.

Figure 6

The positively charged carboxyl terminus of the Fis1p TA is important for specific localization to and insertion at the mitochondrial outer membrane. (A) Deletion of the final five amino acids from the Fis1p TA permits transcriptional activation by Gal4–sfGFP–Fis1p. Strain MaV203, harboring plasmids b100 (WT), b253 (R151X), or b101 (∆TA), was treated as in Figure 5A. (B) Removal of the last five amino acids from the Fis1p TA allows mislocalization to the ER. Strain CDD961, expressing mCherry fused to the WT Fis1p TA from plasmid b109 or expressing mCherry linked to a truncated Fis1p TA (R151X) from plasmid b254, was evaluated as in Figure 2B. (C) Strain CDD961 was cured of plasmid pHS1. The resulting strain was transformed with plasmid pJK59 to label ER by expression of Sec63p–GFP, then transformed with either plasmid b109 or plasmid b254 to localize the WT and R151X TAs, and examined by fluorescence microscopy. Bar, 5 µm.