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Kinetics of the time-dependent inhibition of COX-2 by compound 12. (A) Purified mCOX-2 was reconstituted with heme and pre-incubated with the indicated concentrations of inhibitor at 37 °C for the indicated times prior to the addition of the substrate (50 μM). Data are the mean ± range of duplicate determinations. (B) Secondary plot of kobs versus inhibitor concentration used to generate values for KI, k2, and k−2.
