. 2016 Jul 6;125(2):230–237. doi: 10.1289/EHP258

Table 1.

Blood and brain Mn concentrations in littermates of the behaviorally tested animals (PND 24, 66) and in the behaviorally tested animals at sacrifice (PND ~ 500).

Age (PND)	Control	25 mg Mn/kg/day		50 mg Mn/kg/day
Age (PND)	Control	Early life	Lifelong	Early life	Lifelong
Blood
24	24.2 ± 0.79 (11)^A,a	NA	188 ± 28 (17)^B,a	NA	247 ± 23 (15)^C,a
66	9.51 ± 0.36 (14)^A,b	11.8 ± 0.53 (15)^B	13.7 ± 0.68 (17)^B,^b	13.3 ± 0.78 (15)^B	19.4 ± 1.2 (13)^C,b
490	5.76 ± 0.28 (16)^A,c	7.12 ± 0.56 (21)^A	9.30 ± 0.50 (15)^B,c	6.83 ± 0.36 (16)^A	15.2 ± 1.14 (20)^C,b
Brain
24	3.77 ± 0.19 (11)^A,a	NA	11.3 ± 2.25 (16)^B,a	NA	12.8 ± 1.64 (14)^B^,a
66	2.12 ± 0.031 (14)^A,b	2.24 ± 0.037 (14)^A,B	2.39 ± 0.030 (17)^C,D,b	2.27 ± 0.041 (16)^B,C	2.53 ± 0.047 (14)^D,b
490	1.95 ± 0.063 (13)^A,b	2.05 ± 0.083 (19)^A,B	2.24 ± 0.046 (16)^B,b	1.96 ± 0.051 (12)^A,B	2.63 ± 0.085 (17)^C,b
Note: Data are mean ± standard error (n); blood Mn in ng/mL, brain Mn in μg/g dry weight. A, B, etc. superscripts: within an age group and tissue, treatment groups with different capital letter superscripts are statistically different from one another (p < 0.05), based on Tukey’s post hoc test. Lower case a, b, etc.: within a treatment group and tissue, values across ages with different lower case superscripts are statistically different from one another. Main effect statistics are: blood Mn, age F(2,128) = 764, p < 0.0001, treatment F(2,128) = 198, p < 0.0001, age × treatment F(4,128) = 34.7, p < 0.0001; Brain Mn, F(2,123) = 387, p < 0.0001, treatment F(2,123) = 89.2, p < 0.0001, age × treatment F(4,123) = 20.5, p < 0.0001). PND, postnatal day.