. 2016 Jul 6;125(2):238–245. doi: 10.1289/EHP378

Table 2.

List of differentially expressed proteins in adult mouse spermatozoa following gestational exposure to BPA.

Protein	Symbol	gi Number	Peptide sequence	Matched peptide	Sequence coverage (%)	MASCOT score^a	Relative intensity
Protein	Symbol	gi Number	Peptide sequence	Matched peptide	Sequence coverage (%)	MASCOT score^a	Control^b	TDI^c	NOAEL^d	LOAEL^e
Phospholipid hydroperoxide glutathione peroxidase	PHGPX	gi\|2522259	R.YGPMEEPQVIEK.D	710.35	6.09	66	1.00	0.53 ± 0.18	0.43 ± 0.04*	0.40 ± 0.08*
ATP synthase subunit O, mitochondrial	ATP5O	gi\|20070412	K.VSLAVLNPYIK.R	608.87	5.16	52	1.00	0.34 ± 0.06****	0.40 ± 0.01****	0.45 ± 0.07***
Glutathione S-transferase Mu	GSTM5	gi\|6754086	K.LTFVDFLTYDVLDQN.R	980	7	45	1.00	0.35 ± 0.08**	0.50 ± 0.12**	0.43 ± 0.07**
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial	NDUFA10	gi\|13195624	R.LQSWLYASR.L	562.3	10.11	33.3	1.00	0.50 ± 0.19	0.51 ± 0.17	0.20 ± 0.04*
Isoaspartyl peptidase/ʟ‑asparaginase	ASRGL1	gi\|81875980	K.FAEDMGIPQVPVEK.L	780.4	4.29	65	1.00	3.99 ± 0.17*	4.14 ± 0.72*	3.36 ± 0.43*
Superoxide dismutase [Mn], mitochondrial	SOD2	gi\|31980762	K.GDVTTQVALQPALK.F	720.39	6.3	55	1.00	2.82 ± 0.40**	2.79 ± 0.39*	2.80 ± 0.13**
Notes: BPA, bisphenol A; gi Number, GenInfo Identifier; LOAEL, lowest observed adverse effect level; NOAEL, no observed adverse effect level; TDI, tolerable daily intake value. Relative spot intensity of PHGPX (p < 0.05, compared with control), ATP5O (p < 0.001 and p < 0.0001, compared with control), GSTM5 (p < 0.01, compared with control), NDUFA10 (p < 0.05, compared with control), ASRGL1 (p < 0.05, compared with control), and SOD2 (p < 0.05 and *p < 0.01, compared with control) are presented as the mean ± SEM (n = 3 mice/replicate). Data were analyzed by one-way analysis of variance (ANOVA) with multiple comparisons, with Tukey’s multiple comparison test. ^aMASCOT score is –log₁₀(p), where p is the probability that the observed match is a random event. Individual scores > 30 indicate identity or extensive homology (p < 0.05). ^bCorn oil. ^cTDI = 50 μg/kg bw/day. ^dNOAEL = 5 mg/kg bw/day. ^eLOAEL = 50 mg/kg bw/day.