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. Author manuscript; available in PMC: 2017 Sep 16.
Published in final edited form as: ACS Chem Biol. 2016 Jul 11;11(9):2438–2446. doi: 10.1021/acschembio.6b00397

Figure 4. GC-MS traces for hydrolyzed and derivatized (Me)Lan residues from CylLS and CylLS–T2A peptides that were cyclized using different set ups.

Figure 4

Gas chromatograms showing signals corresponding to derivatized MeLan (left panel) and Lan (right panel) from CylLS peptides cyclized by CylM (black trace)24, by the CylM cyclase domain (blue trace) or without a cyclase (brown trace), and CylLS–T2A peptide cyclized by CylM (magenta trace) are overlaid. For co-injection traces with synthetic (Me)Lan standards that were used for stereochemistry assignments, see Supporting Information Figures 4, 14 and 17.