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. 2017 Jan 25;4(1):84–96.e6. doi: 10.1016/j.cels.2016.11.015

Figure 7.

Figure 7

YAP Regulation Is Uncoupled from Cell-ECM Adhesion and β-PIX-Associated Genes in Metastatic Breast Tumor Cells

(A–D) Average nuclear/cytoplasmic YAP ratios in replicate wells of wild-type (mock-transfected) and siRNA-transfected cells from four variants of MDA-MB-231 cells plotted as a function of cell-cell contact area (NF). (A) Parental cell population. (B) Parental cells selected to stably express GFP. (C) D3H2LN cells harvested from mouse lymph node metastasis. (D) D3H2LN cells selected by passage through 3 micron pores. Replicate well averages are shown (n = 150–2,500 cells/well).

(E) Cell areas. Mean ± SD of replicate wells (n = 6 wells/condition). ∗p < 0.01 versus wild-type for each cell line.

(F) Focal adhesions labeled by anti-paxillin immunofluorescence in representative fields of wild-type (top) and siRNA-transfected cells (bottom). Scale bar, 20 μm.