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. 2017 Jan 27;174(4):328–340. doi: 10.1111/bph.13693

Figure 4.

Figure 4

Identifying IGF‐1 receptors inhibitory antibodies with activity against M22 stimulation. (A) Cultured GOF cells were stimulated with IGF‐1 EC50 and co‐treated with anti‐IGF‐1 receptor inhibitory antibodies AF305 or 1H7 at 1 μg·mL−1 concentrations in comparison to 1 μM linsitinib (Lins [ICmax]) for 4 days. Total HA was measured in culture media by elisa. Data bars represent mean ± SEM from three donor cell strains plotted as percent HA levels relative to the M22 median response. (B) Cultured GOF cells were stimulated with M22 ECmed and co‐treated with anti‐IGF‐1 receptor blocking antibodies AF305 or 1H7 at the indicated concentrations for 4 days. Total HA was measured by elisa as described in (A). Data represent mean ± SEM from three donor cell strains plotted as percent HA levels relative to the M22 median response. (C) Cultured GOF cells were stimulated with M22 ECmed and treated with increasing doses of 1H7 for 5 days. Total HA was measured by elisa assay as described in (A). Data represent mean ± SEM of five donor cell strains plotted as percent HA levels relative to the M22 median response (0% corresponding to HA levels at baseline). Indicated is the approximate 1H7 IC50 concentration (14.3 nM). (D) GOF cells were treated with maximal doses (ICmax) of linsitinib (1 μM) or 1H7 (0.3 μM) and stimulated with a maximal concentration (ECmax) of M22 (10 nM). Total HA was measured in culture media by elisa. Data represent mean ± SEM of five donor cell strains plotted as percent HA levels relative to maximal response. * P <0.001, significantly different from M22 [ECmax].