Skip to main content
. 2017 Feb 3;8:66. doi: 10.3389/fimmu.2017.00066

Figure 4.

Figure 4

Lymphatic endothelial cells (LECs) upregulate PDL1 in response to IFN-g. (A) Immortalized mouse LECs (imLECs) were treated with VEGF-A (20 ng/ml), VEGF-C (200 ng/ml), TNF-a (40 ng/ml), or IFN-g (100 ng/ml). Expression of PDL1 was assessed by qPCR after 6, 24, and 48 h. Incubation with IFN-g resulted in a significant upregulation of PDL1 mRNA (pooled data of three individual experiments are shown). (B) Example histogram of surface PDL1 expression assessed by FACS in imLECs treated with TNF-a or IFN-g or not (Ctr). (C) Quantification of surface PDL1 in untreated (Ctr), TNF-a treated, and IFN-g treated imLECs (N = 3). (D) qPCR showing PDL1 expression upon imLEC treatment with tumor cell conditioned media (Cond med) derived from B16F10-VEGFC or 4T1 cells, compared to control media (Ctr med) (pooled data of three individual experiments are shown). (E) Example histograms of surface PDL1 expression assessed by FACS in imLECs treated with B16F10-VEGFC (left) or 4T1 cell conditioned medium (right). (F) Quantification of surface PDL1 expression in imLECs treated with control (Ctr med) or tumor cell conditioned media (Cond med) (N = 3). (G,H) qPCR data showing IFN-g expression in B16F10-VEGFC (G) and 4T1 tumor tissue (H) compared to control tissue (back skin resp. abdominal skin) (N = 7–9 for B16F10-VEGFC and 4–7 for 4T1).