Table 3.

Comparison of viability status profiling results from FCM with classical culture-based technique (Micro) for E. faecalis treated with various disinfectants.

Bacteria	Product	No. trials	Dilution*	FCM	Micro	Positive tests (+) FCM	Positive test (+) MICRO
E. faecalis	Sodium Hypochloride	1	1/16	+	−	13/13	7/13
		2	1/16	+	−
		3	1/16	+	−
		4	1/32	+	−
		5	1/32	+	−
		6	1/32	+	+
		7	1/64	+	+
		8	1/64	+	−
		9	1/64	+	+
		10	1/128	+	+
		11	1/128	+	+
		12	1/128	+	+
		Ctrl	−	+	+
	Accelerated hydrogen Peroxide based (bactericide)	1	1/32	−	−	4/7	4/7
		2	1/32	−	−
		3	1/32	−	−
		4	1/64	+	+
		5	1/64	+	+
		6	1/64	+	+
		Ctrl	−	+	+
	Accelerated hydrogen Peroxide based (tuberculocide)	1	1/64	−	−	4/7	4/7
		2	1/64	−	−
		3	1/64	−	−
		4	1/128	+	+
		5	1/128	+	+
		6	1/128	+	+
		Ctrl	−	+	+
	Phenol based	1	1/8	−	−	4/7	4/7
		2	1/8	−	−
		3	1/8	−	−
		4	1/16	+	+
		5	1/16	+	+
		6	1/16	+	+
		Ctrl	−	+	+
	Quaternary ammonium 3rd generation	1	1/64	+	+	8/10	8/10
		2	1/64	−	−
		3	1/64	−	−
		4	1/128	+	+
		5	1/128	+	+
		6	1/128	+	+
		7	1/256	+	+
		8	1/256	+	+
		9	1/256	+	+
		Ctrl	−	+	+
	Quaternary ammonium 4th generation	1	1/64	+	+	8/10	8/10
		2	1/64	−	−
		3	1/64	−	−
		4	1/128	+	+
		5	1/128	+	+
		6	1/128	+	+
		7	1/256	+	+
		8	1/256	+	+
		9	1/256	+	+
		Ctrl	−	+	+

+, Live cells; −, Dead cells; Ctrl, Without disinfectant.

^*Dilution performed further to manufacturer recommended working dilution.