Table 1.

Summary of the miRNA genes successfully edited by the CRISPR/Cas9 system.

miRNA	Chromosomal location	Cells/Animals tested	Results	Reference
miR-21	Chr17	Human HEK293, LNCaP, MCF-7 and HCT-116 cells	The miR-21 gene was knocked out with over 90% reduction of the miR-21 levels	Ho et al. (2015)
miR-29a	Chr7	Human HEK293 cells	Complete knock-out of the miR-29a gene	Ho et al. (2015)
miRNA cluster (miR-17a-1, miR-92a-1)	Chr1	Zebrafish	The deletion of this miRNA cluster gene from dre-miR-17a-1 to dre-miR-92a-2 was obtained in the zebrafish embryos injected with the CRISPR/Cas9 in combination with the gRNAs	Xiao et al. (2013)
miR-17	Chr13	Human HCT-116 and HT-29 cells	The miR-17 expression was decreased by up to 96% and the knock-down phenotype was stable in the human cells and mice	Chang et al. (2016)
		Mice
miR-141	Chr12	Human HCT-116 and HT-29 cells	Nearly 96% reduction in the miR-141 levels was achieved with long-term stability of the knock-down phenotype and in control of crossing off-target effect on other members in the same family	Chang et al. (2016)
		Mice