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. 2017 Feb 3;8:91. doi: 10.3389/fimmu.2017.00091

Figure 3.

Figure 3

Protein detection by using Western blot, ELISA analysis, and flow cytometry. (A) Western blot of HDFs lysates confirms mRNA expression of IL-6, IL-8, and CXCL-1 at the protein level, although shows no significant increase in IL-8 protein of UVB group compared with Control group and no significant decrease in IL-6 and CXCL-1 protein of IL-17A/TNF-α + UVB group compared with IL-17A/TNF-α group. (B) ELISA analysis of the supernatant confirms mRNA expression of IL-6, IL-8, and CXCL-1 at the protein level. (C) The expression of IL-17RA and IL-17RC mRNA is confirmed by flow cytometry for detection of the surface expression of IL-17RA and IL-17RC on HDFs. The data (fold change) of Western blot are expressed as the mean ± SD of three independent experiments. Other data are from one representative experiment performed in triplicate, repeated three times with similar results. UVB, ultraviolet B; HDFs, human dermal fibroblasts. Statistical significance indicated: *P < 0.05, **P < 0.01, ***P < 0.001.