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. 2017 Feb 3;8:91. doi: 10.3389/fimmu.2017.00091

Table 1.

Primers for the target genes in real-time quantitative PCR.

Target genes Primers
GAPDH (115 bp) Sense: 5-CATCTTCTTTTGCGTCGCCA-3
Antisense: 5-TTAAAAGCAGCCCTGGTGACC-3
IL-6 (120 bp) Sense: 5-GACAGCCACTCACCTCTTCA-3
Antisense: 5-CCTCTTTGCTGCTTTCACAC-3
IL-8 (149 bp) Sense: 5-GCAGAGGGTTGTGGAGAAGT-3
Antisense: 5-CCCTACAACAGACCCACACA-3
CXCL-1 (126 bp) Sense: 5-AATCCAACTGACCAGAAGGG-3
Antisense: 5-CATTAGGCACAATCCAGGTG-3
TNFR-1 (98 bp) Sense: 5-TACCGGCATTATTGGAGTGA-3
Antisense: 5-GTGTTCTGTTTCTCCTGGCA-3
TNFR-2 (109 bp) Sense: 5-ACACCGTGTGTGACTCCTGT-3
Antisense: 5-TGAGTTTCCACCTGGTCAGA-3
IL-17RA (128 bp) Sense: 5-GCTGCCTTTGTCCTCCTAAC-3
Antisense: 5- GACTGACTGTGCTGATGGCT -3
IL-17RC (84 bp) Sense: 5-CAGAAGGAGACCGACTGTGA-3
Antisense: 5-CTCATCTTCAGGCTCTTCCC-3
TGF-β1 (128 bp) Sense: 5-AACCGGCCTTTCCTGCTTCT-3
Antisense: 5-CGCACGCAGCAGTTCTTCTC-3
Smad3 (85 bp) Sense: 5-ATGCAGCAGTGGAGCTGACA-3
Antisense: 5-AGGCACTCTGCGAAGACCTC-3