FIGURE 1.

Determination of gene order in recovered viral genomes. (A) Schematic representations of the gene order of rHep-Flury and the rearranged variant viruses. (B) Genomic RNA was isolated from recovered viruses and analyzed by reverse transcription and PCR with different primer pair. Fragments were analyzed by electrophoresis on a 1% agarose gel in the presence of ethidium bromide. Lane M = marker DNA fragments with sizes as indicated. Lanes 1, 4, 7, 10, and 13: PCR products with primers D–F and P–R; Lanes 2, 5, 8, 11, and 14: PCR products with primers D–F and N–R; Lanes 3, 6, 9, 12, and 15: PCR products with primers D–F and M–R.