FIGURE 2.

Salt treatment induces loss of viability in tomato roots. (A) Experimental assay. Tomato (Solanum lycopersicum) plants were grown in a hydroponic system and 5-weeks old plants were treated with 0-, 100- and 250 mM NaCl in the nutrient solution. Root tips were collected at 1, 6, and 24 h. (B) Loss of viability upon salt stress. Root tips were stained with fluorescein diacetate (FDA) to detect the viable cells. Top: representative images are shown. Scale bar, 0.5 mm. Bottom: fluorescent intensities of FDA fluorescence levels, when compared to the control. Error bars represent SEM of n = 3 biological replicates.