FIGURE 6.

Subunit-specific labeling confirms modification of β5. (A) Tomato roots were treated with 0- and 250 mM NaCl and root extracts were generated after 6 h and pre-incubated with or without subunit-specific inhibitors, N3β1 and N3β5 and co- labeled with or without subunit-selective probes, LW124 (β1) and MVB127 (β5). Samples were separated on 1D gel. Differential signals are indicated by black arrowheads. (B) Tomato roots were treated with 0- and 250 mM NaCl and root extracts were generated after 6 h and co-labeled with LW124 (β1) and MVB127 (β5) and separated on IEF 2D gel. Differential signals are indicated by black arrowheads.