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. 2017 Feb 3;8:107. doi: 10.3389/fpls.2017.00107

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Copyright © 2017 Kovács, Poór, Kaschani, Chandrasekar, Hong, Misas-Villamil, Xin, Kaiser, Overkleeft, Tari and van der Hoorn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phosphatase and glycosidase treatments do not affect altered proteasome activity profile. (A) Root extracts of 250 mM treated samples, labeled by MVB072 were treated with alkaline phosphatase at different conditions. (B) Dephosphorylation of MAP kinase, used as a positive control, detected by an anti-phosphoMAPK antibody. (C) Root extracts of 250 mM treated-samples, labeled by MVB072 were treated with and without PNGase F or deglycosylation mix. (D) Enzymatic deglycosylation of Bovine Fetuin was used as a positive control.