Table 1.
Primer name | Sequence (5′–3′) |
---|---|
tps46 fragment clone | |
RiTPS46-forward | TCAggtaccactagtCTACACGAAATGAAGCCATA |
RiTPS46-reverse | CATggatccgagctcAGTTCCAGGTGTTCCTCTAT |
tps46 gene clone | |
OeTPS46-forward | CATGTCATCGACACCTGCA |
OeTPS46-reverse | TTAAATGCTATATGGCTCAACG |
qRT-PCR | |
TPS46-forward | TGAAGAGGCACTAGGTCCAAAC |
TPS46-reverse | CCATCCCAACTAAAGAAGCACA |
EF1α-forward | AGACGCACATCAACATCG |
EF1α-reverse | GAACTTCCACAGGGCAATA |
Race-PCR | |
TPS46-3′GSP1 | CACACGATGGTGGAAAGAGCTTAACGTTG |
TPS46-3′GSP2 | GACAGGAGCATGCTCGGAGCCCCATTA |
TPS46-5′GSP1 | CAAGCATCATGCTCTCCTCGGTTGTAGC |
TPS46-5′GSP2 | TGGACCTAGTGCCTCTTCAAATGAATCAA |
The restriction sites used for clone are underlined and shown in bold.