Table 1.
Strains used in this study.
| Strain | Genotype or phenotype | Defective in the production of | References or comments |
|---|---|---|---|
| Pseudomonas protegens | |||
| CHA0 | Wild type, isolated from tobacco roots | Stutz et al., 1986; Jousset et al., 2014 | |
| CHA19 | ΔgacS deletion mutant of CHA0 | GacS sensor | Zuber et al., 2003 |
| CHA89 | gacA::Kmr insertion mutant of CHA0 | GacA response regulator | Laville et al., 1992 |
| CHA400 | pvd-400::Tn1733 insertion mutant of CHA0; Kmr | Pyoverdine (Pvd) | Keel et al., 1989 |
| CHA805 | Nonpolar aprA::'lacZ insertion mutant of CHA0 | Protease AprA (AprA) | Siddiqui et al., 2005 |
| CHA805-g | CHA805::attTn7-gfp2, Gmr | AprA | This study |
| CHA1151 | ΔfitD in-frame deletion mutant of CHA0 | Fit toxin (Fit) | Péchy-Tarr et al., 2008 |
| CHA1196 | Δgcd in-frame deletion mutant of CHA0 | Glucose dehydrogenase (Gcd) | De Werra et al., 2009 |
| CHA1197 | Δgad in-frame deletion mutant of CHA0 | Gluconate dehydrogenase (Gad) | De Werra et al., 2009 |
| CHA1241 | ΔphlACBD deletion mutant of CHA0 | 2,4-Diacetylphloroglucinol (Phl) | This study |
| CHA1281 | ΔfitD gacA::Kmr mutant of CHA0 | Fit toxin and GacA | Ruffner et al., 2013 |
| CHA5091 | ΔprnABCD deletion mutant of CHA0 | Pyrrolnitrin (Prn) | This study |
| CHA5092 | ΔpltABCDEFG deletion mutant of CHA0 | Pyoluteorin (Plt) | This study |
| CHA5098 | ΔphlACBD ΔprnABCD ΔpltABCDEFG deletion mutant of CHA0 | Phl/Prn/Plt | This study |
| CHA5101 | ΔofaABC deletion mutant of CHA0 | Orfamide (Ofa) | This study |
| CHA5103 | ΔhcnABC deletion mutant of CHA0 | Hydrogen cyanide (Hcn) | This study |
| CHA5118 | ΔphlACBD ΔprnABCD ΔpltABCDEFG - ΔhcnABC ΔofaABC deletion mutant of CHA0 | Phl/Prn/Plt/Hcn/Ofa | This study |
| Pseudomonas chlororaphis | |||
| PCL1391 | Wild type, isolated from tomato roots | Chin-A-Woeng et al., 1998 | |
| PCL1113 | phzF:: Tn5-luxAB insertion mutant of PCL1391; Kmr | Phenazine-1-carboxylic acid and phenazine-1-carboxamide (Phz) | Chin-A-Woeng et al., 1998 |
| PCL1123 | gacS::Tn5-luxAB insertion mutant of PCL1391; Kmr | GacS | This study |
| PCL1832 | PCL1391_3318::Tn5-luxAB insertion mutant of PCL1391; Kmr | Clp1391 | This study |
| PCL5103 | ΔhcnABC deletion mutant of PCL1391 | Hcn | This study |
| Pseudomonas sp. | |||
| CMR12a | Wild type, isolated from cocoyam roots | Perneel et al., 2007 | |
| CMR12a-ΔPhz | ΔphzABCDEFGH deletion mutant of CMR12a | Phenazine (Phz) | D'aes et al., 2011 |
| CMR12a-Clp1 | sesA insertion mutant, Gmr | Sessilin (Ses) | D'aes et al., 2011 |
| CMR12a-ΔPhz-Clp1 | ΔphzABCDEFGH sesA mutant of CMR12, Gmr | Phz/Ses | D'aes et al., 2011 |
| CMR12a-ΔClp2 | ΔofaBC deletion mutant of CMR12a | Orfamide (Ofa) | D'aes et al., 2014 |
| CMR12a-ΔPhz-ΔClp2 | ΔphzABCDEFGH ΔofaBC mutant of CMR12a | Phz/Ofa | D'aes et al., 2014 |
| CMR12a-ΔClp2-Clp1 | ΔofaBC sesA mutant of CMR12a, Gmr | Ofa/Ses | D'aes et al., 2014 |
| CMR12a-ΔPhz-ΔClp2-Clp1 | ΔphzABCDEFGH ΔofaBC sesA mutant of CMR12a, Gmr | Phz/Ofa/Ses | D'aes et al., 2014 |
| Escherichia coli | |||
| DH5α | recA1 endA1 hsdR17 deoR thi-1 supE44 gyrA96 relA1 Δ(lacZYA-argF)U169(Φ80dlacZΔM15) | Sambrook and Russel, 2001 | |
| S17-1/λpir | pro thi hsdR recA chromosome::RP4-2; Tc::Mu Km::Tn7/ λpir; Tpr Smr | Simon et al., 1983 | |
Gmr, gentamicin; and Kmr, kanamycin resistance, respectively.