Table 2.
Lethal time 50 (LT50) for Galleria mellonella larvae injected with wild-type and mutant Pseudomonas strains.
| Strain | Phenotype | LT50 (h) | |||
|---|---|---|---|---|---|
| Experiment 1 | Experiment 2 | Experiment 3 | Experiment 4 | ||
| CHA0 | Wild type | 29.1 (28.8; 29.4) | 32.9 (32.5; 33.3) | 33.8 (33.4; 34.1) | 33.7 (33.3; 34.1) |
| CHA1151 | Fit− | 30.6 (30.1; 31.1)* | 32.8 (32.5; 33.2) | 34.6 (34.2; 35.0)* | 36.3 (35.8; 36.7)* |
| CHA1281 | GacA−Fit− | 31.8 (31.3; 32.2)* | 35.3 (34.6; 35.9)* | 34.1 (33.7; 34.5) | 36.8 (36.3; 37.3)* |
| CHA400 | Pvd− | 33.0 (32.4; 33.6) | 34.1 (33.6; 34.5) | ||
| CHA1196 | Gcd− | 34.7 (34.0; 34.8) | 34.7 (34.2; 35.2)* | ||
| CHA1197 | Gad− | 33.9 (33.4; 34.3) | 30.4 (29.8; 30.9)† | ||
| CHA805-g | AprA− | 33.6 (33.3; 34.0) | 32.7 (32.3; 33.0)† | ||
| CHA1241 | Phl− | 28.7 (28.3; 29.1) | 33.0 (32.6; 33.5) | ||
| CHA5091 | Prn− | 28.5 (28.2; 28.9) | 32.7 (32.3; 33.0) | ||
| CHA5092 | Plt− | 28.6 (28.3; 29.0) | 30.8 (30.3; 31.3)† | ||
| CHA5101 | Ofa− | 28.2 (27.6; 28.8) | 30.9 (30.3; 31.4)† | ||
| CHA5103 | Hcn− | 30.9 (30.5; 31.2)* | 35.8 (35.4; 36.2)* | ||
| CHA5118 | Phl−Prn−Plt−Hcn−Ofa− | 29.3 (28.9; 29.8) | 32.6 (32.1; 33.1) | ||
| CHA19 | GacS− | 36.0 (35.3; 36.7)* | 32.0 (31.5; 32.6)† | ||
| CHA89 | GacA− | 32.6 (32.2; 33.1)† | 33.8 (33.3; 34.3) | ||
| Control | >40 | >40 | >38 | >38 | |
| Experiment 1 | Experiment 2 | ||||
| CMR12a | Wild type | 26.8 (26.5; 27.0) | 29.2 (28.6; 29.8) | ||
| Clp1 | Ses− | 29.4 (29.1; 29.7)* | 34.3 (33.9; 34.7)* | ||
| ΔClp2 | Ofa− | 26.4 (26.2; 26.7) | 28.2 (27.8; 28.7) | ||
| ΔClp2-clp1 | Ofa− Ses− | 27.0 (26.7; 27.3) | 30.0 (29.7; 30.4) | ||
| ΔPhz | Phz− | 26.7 (26.5; 27.0) | 29.8 (29.5; 30.2) | ||
| ΔPhz-Clp1 | Phz−Ses− | 28.5 (28.2; 28.7)* | 32.0 (31.7; 32.4)* | ||
| ΔPhz-ΔClp2 | Phz−Ofa− | 26.1 (25.9; 26.3)† | 29.1 (28.6; 29.6) | ||
| ΔPhz-ΔClp2-Clp1 | Phz−Ofa−Ses− | 28.8 (28.4; 29.2)* | 31.9 (31.5; 32.3)* | ||
| Control | >39 | >39 | |||
| Experiment 1 | Experiment 2 | Experiment 3 | Experiment 4 | ||
| PCL1391 | Wild type | 28.3 (28.0; 28.6) | 29.3 (29.0; 29.7) | 28.9 (28.4; 29.4) | 26.5 (26.2; 26.8) |
| PCL1832 | Clp1391− | 30.0 (29.6; 30.4)* | 32.3 (31.8; 32.7)* | 31.2 (30.7; 31.4)* | 28.3 (27.8; 28.7)* |
| PCL1113 | Phz− | 28.8 (28.4; 29.1) | 30.0 (29.6; 30.5) | ||
| PCL1123 | GacS− | 29.2 (28.9; 29.5)* | 30.5 (30.0; 31.0)* | ||
| PCL5103 | Hcn− | 32.4 (31.8; 33.0)* | 27.4 (27.1; 27.8)* | ||
| Control | >39 | >39 | >33.5 | >34.5 | |
Lethal time (LT50) values were calculated based on a generalized linear model (MASS package in R) from three replicates with 10 larvae per replicate. Confidence intervals (95%) for LT50 values are given in brackets. Marked values differed significantly from the LT50 value of the respective wild-type strain (p < 0.05).
significantly faster;
significantly slower. If 50% mortality was not reached by the end of the experiment, LT50 values are given as >X, with X being the last time point at which the larvae were checked for survival. Sterile 0.9% NaCl served as control (Control). Each mutant was tested at least three times and results of at least two representative experiments are depicted. AprA, extracellular protease; Clp1391, cyclic lipopeptide of strain PCL1391; Fit, P. fluorescens insecticidal toxin; Gad, gluconate dehydrogenase; Gcd, glucose dehydrogenase; Hcn, hydrogen cyanide; Ofa, orfamide; Phl, 2,4-diacetylphloroglucinol; Phz, phenazine; Plt, pyoluteorin; Prn, pyrrolnitrin; Pvd, pyoverdine; Ses, sessilin.