Table 2.

Summary of Cell Density Measurements

Cell density	Total (N = 29) Mean, cells/mm2; median, cells/mm2 (range)	Primary (n = 12) Mean, cells/mm2; median, cells/mm2 (range)	Metastatic (n =17) Mean, cells/mm2; median, cells/mm2 (range)	Significance between primary and metastatic (P)
CD8 (invasive margin)	107.9; 44.1 (0–730)	73.0; 9.9 (0–730)	132.5; 100.3 (23.1–357.1)	0.0003
PD-1 (invasive margin)	82.5; 48.9 (1.8–361.6)	41.6; 8.9 (1.8–293.2)	111.3; 83.4 (16.2–361.6)	0.002
PD-L1 (invasive margin)	174.2; 113.9 (0–1035)	80.0; 5.7 (0–862.8)	240.6; 194.5 (15.8–1035)	0.0003
CD8 (intratumor)	53.4; 15.0 (1.3–414.3)	26.2; 8.1 (2.2–204.7)	72.6; 36.9 (1.3–414.3)	0.11
PD-1 (intratumor)	36.9; 11.1 (1.1–309)	24.8; 5.3 (1.1–182.9)	45.5; 20.9 (1.5–309)	0.08
PD-L1 (intratumor)	173.3; 24.3 (1.5–1940)	171.3; 5.6 (1.5–1940)	174.7; 53.7 (1.6–1177)	0.006
NY-ESO-1	5024; 5334 (566–9170)	4682; 5050 (566–8693)	5265; 5920 (946–9170)	0.44