Table 1. Primer sequences for real-time PCR.
Primers | Accession No. | Sequences (5' → 3') | Size, bp | Cycle No. | Annealing temperature, ℃ |
---|---|---|---|---|---|
HMGB1 | NM012963 | F: GGCGAGCATCCTGGCTTATC | 142 | 35 | 60 |
R: AGGCAGCAATATCCTTCTCATAC | |||||
GAPDH | NM0170084 | F: GCACAGTCAAGGCTGAGAATG | 142 | 35 | 60 |
R: ATGGTGGTGAAGACGCCAGTA |
PCR = polymerase chain reaction, HMGB1 = high mobility group box1, GAPDH = glyceraldehyde-3-phosphate dehydrogenase.