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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Mol Cancer Res. 2016 Nov 17;15(2):128–140. doi: 10.1158/1541-7786.MCR-16-0270-T

Figure 5. Cells isolated from tumors of mice in the treated group showed impaired sphere formation.

Figure 5

Cells were isolated from tumors of mice in the control (n=3) and Ko143 group (n=3) by protease digestion and plated in 24 well ultralow attachment plates coated with matrigel at a density of 4000 cells/well. On day 14, spheres were (a) imaged under 20X magnification, (b) sphere sizes were measured by the basic SPOT software and (c) spheres were counted. The student’s t test was used for statistical analysis (p**<0.01; ****p<0.0001).