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. 2017 Jan 27;8:14056. doi: 10.1038/ncomms14056

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Stress–strain relation for PAA beads prepared by the ratio of 5/0.225 acrylamide/bisacrylamide. The linear fit has been determined for the first three points. We used and fitted polynomial Rivlin–Mooney model to determine the strain in the non-linear region. Results are represented by mean±s.d. Inset: fluorescent images of a bead at the initial state P=0 kPa and after application of a mechano-osmotic stress P=5 kPa. Scale bar, 20 μm.