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. 2017 Jan 27;8:14186. doi: 10.1038/ncomms14186

Figure 1. Depletion of DDRGK1 leads to apoptosis and elevated ER stress.

Figure 1

(a) MCF7 and HepG2 cells were transfected with either control siRNA or siRNA targeting DDRGK1 for 72 h. The cells were subsequently stained with Annexin V and PI and subjected to flow cytometric analysis followed by the quantification of apoptotic cells (Annexin V+). (b) Western blot analysis of PARP and cleaved caspase-3 in control and DDRGK1-knockdown MCF7 and HepG2 cells described in a. (c,d) Q-PCR analysis of the relative mRNA expression levels of BAX, BAK, NOXA, Bid, DR-5 and Bcl-2 in control and DDRGK1-knockdown MCF7 and HepG2 cells. (e,f) Q-PCR analysis of the relative mRNA expression levels of BiP, HSPA8 and CHOP in control and DDRGK1-knockdown MCF7 and HepG2 cells. All data are presented as mean±s.d. from three experiments. *P<0.05, **P<0.01 and ***P<0.001 by Student's t-test.