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. 2017 Jan 27;8:14186. doi: 10.1038/ncomms14186

Figure 2. DDRGK1 plays a protective role in ER stress-induced apoptosis.

Figure 2

(a). HepG2 cells were transfected with control siRNA or with siRNA against DDRGK1 for 72 h, and then the cells were treated with DMSO (vehicle control) or Tg (2.5 μM) for 24 h before harvesting. The cells were stained with Annexin V and PI, followed by flow cytometric analysis. (b). Quantification of the apoptotic cells (Annexin V+) in a. (c) Western blot analysis of PARP and cleaved caspase-3 in the HepG2 cells described in a. (d) HepG2 cells were transfected with control vector or DDRGK1 for 36 h, and the cells were treated with DMSO or Tg (2.5 μM) for 24 h before harvesting. The cells were stained with Annexin V and PI, followed by flow cytometric analysis. (e) Quantification of the apoptotic cells (Annexin V+) in d. (f) Western blot analysis of PARP and cleaved caspase-3 in the HepG2 cells described in d. All data are presented as mean±s.d. from three experiments. **P<0.01 and ***P<0.001 by Student's t-test.