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. 2017 Jan 27;8:14186. doi: 10.1038/ncomms14186

Figure 6. Ufmylation is required for the interaction between DDRGK1 and IRE1α.

Figure 6

(a) MCF7 cells were transfected with either control siRNA or siRNA targeting Ufm1 for 72 h. The protein levels of IRE1α were determined by western blot. (b) Western blot analysis of IRE1α in control and Ufm1-knockdown MCF7 cells expressing vector or DDRGK1. (c) The protein level of IRE1α was determined by western blot in control and Ufm1-knockdown MCF7 cells after treatment with 2.5 μM Tg for the indicated times. (d) Western blot analysis of Flag M2 affinity gel immunoprecipitates in HEK293T control and Ufm1-knockdown cells expressing Flag-Vector or Flag-DDRGK1. (e) Western blot analysis of Flag M2 affinity gel immunoprecipitates in HEK293T cells transfected with Flag-Vector, Flag-DDRGK1 WT or K267R mutant. (f) Western blot analysis of IRE1α in MCF7 cells transfected with Flag-Vector, Flag-DDRGK1 WT or K267R mutant.