Skip to main content
. 2017 Jan 27;8:14186. doi: 10.1038/ncomms14186

Figure 8. Knockdown of DDRGK1 induces ER stress in HSCs.

Figure 8

(a) Representative FACS pattern showing the percentage of Annexin V-positive cells within the donor-derived control and DDRGK1-knockdown LSK cells after transplantation (Left panel). Bar graph shows the percentage of Annexin V-positive cells within LSK cells (Right panel, n=3). Data are presented as mean±s.d. *P<0.05 by Student's t-test. (b) Q-PCR analysis of the relative mRNA expression levels of DDRGK1, BiP and CHOP in donor-derived control and DDRGK1-knockdown LSK cells after transplantation (n=3). Data are presented as mean±s.d. **P<0.01 and ***P<0.001 by Student's t-test. (c) Western blot analysis of p-IRE1α, IRE1α, p-PERK, PERK and ATF6 in donor-derived GFP+Lineage-c-Kit+ cells enriched from control or DDRGK1-knockdown recipient mice. *Represents non-specific band. (d) RT-PCR analysis of XBP-1 splicing in donor-derived control and DDRGK1-knockdown LSK cells after transplantation. MEF cells with Tg treatment served as an XBP-1 splicing control.