MYC promotes mRNA cap methylation of canonical Wnt signaling pathway transcripts through TRRAP-containing cofactor complexes by utilizing RNMT. (A) m7G-mRNA levels of Wnt signaling transcripts in IMEC cells following siRNA depletion of RNGTT, RNMT, RAM, or MYC (normalized to total mRNA input). mRNA levels of each factor following siRNA knockdown in right panel (normalized to ACTB) (n=3, biological). (B) m7G-mRNA levels of Wnt signaling transcripts in IMECs following transient transfection with HA-RNMT, FLAG-RAM, HA-RNMT and FLAG-RAM, or CBP-MYC (normalized to total mRNA input). Protein levels of each factor following transfection in right panel (n=2, biological). (C) m7G-mRNA levels of Wnt signaling transcripts in IMEC cells following siRNA depletion of TIP60 or TRRAP (normalized to total mRNA input). Protein levels of each factor following siRNA knockdown in right panel (n=3, biological). Error bars indicate SEM and asterisks indicate significance (*p < 0.05, **p < 0.01, ****p < 0.0001).