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. 2017 Jan 27;8:14182. doi: 10.1038/ncomms14182

Figure 1. Expression of BCAS2 in male mouse germ cells.

Figure 1

(a) Immunofluorescence (IF) staining of BCAS2 in the paraffin sections of testes from E15.5 to P14 mice. The DNA was stained with Hoechst 33342. Scale bar, 50 μm. (b) Real-time PCR analysis of Bcas2 expression in the fraction of spermatogenic cells (FSPCs) and the fraction of somatic cells (FSCs) enriched from P9 testes. Gapdh was used as the internal control for normalization (n=4). Error bars represent s.e.m. (c) Western blotting analysis of BCAS2 expression in the fraction of spermatogenic cells (FSPCs) and the fraction of somatic cells (FSCs) enriched from P9 testes. Germ cell markers (DAZL and MVH) were used as the indicator of the enrichment efficiency and α-tubulin was used as the loading control. (d) Paraffin sections of P8 testes were co-stained with rabbit anti-BCAS2 and mouse anti-PLZF antibodies. The DNA was stained with Hoechst 33342. Scale bar, 20 μm.